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In vivo characterization of Debio 1562M in mice and cytotoxicity assessment in human PBMCs (A) Concentrations of total ADC and total antibody in the plasma of healthy mice were measured over time following i.v. injection with 1 mg/kg Debio 1562M ( n = 3; mean ± SEM). (B) Plasma concentrations of Debio 1562 or Debio 1562M were monitored over time in tumor-bearing mice following i.v. injection with 10 mg/kg of either ADC ( n = 3; mean ± SEM). (C–G) Male and female mice were injected with vehicle (control) or DAR-equivalent doses of Debio 1562 (single dose, 100 mg/kg) or Debio 1562M (every 3 weeks, Q3W ×2, 50 mg/kg doses) ( N ≥ 5; mean ± SEM). (C) Body weights were monitored throughout the experiment. (D) Platelets, (E) WBC counts, and (F) liver enzymes (shown are ALP, alanine transferase [ALT], and aspartate transferase [AST] levels) were measured on day 5 (Debio 1562 experiments), day 11 (Debio 1562M single dosing experiments), or day 23 (Debio 1562M Q3W ×2 dosing); two-way ANOVA p values: ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (G) PBMCs from healthy volunteers were cultured in the presence of vehicle or 1 μM Debio 1562M for 72 h prior to immunophenotyping and assessment of viability. n = 5; mean ± SEM. Two-way ANOVA p values: ∗ p < 0.05, ns: not significant. See also .

Journal: Cell Reports Medicine

Article Title: Debio 1562M CD37-targeting ADC is highly active and well tolerated in preclinical models of AML and MDS

doi: 10.1016/j.xcrm.2026.102749

Figure Lengend Snippet: In vivo characterization of Debio 1562M in mice and cytotoxicity assessment in human PBMCs (A) Concentrations of total ADC and total antibody in the plasma of healthy mice were measured over time following i.v. injection with 1 mg/kg Debio 1562M ( n = 3; mean ± SEM). (B) Plasma concentrations of Debio 1562 or Debio 1562M were monitored over time in tumor-bearing mice following i.v. injection with 10 mg/kg of either ADC ( n = 3; mean ± SEM). (C–G) Male and female mice were injected with vehicle (control) or DAR-equivalent doses of Debio 1562 (single dose, 100 mg/kg) or Debio 1562M (every 3 weeks, Q3W ×2, 50 mg/kg doses) ( N ≥ 5; mean ± SEM). (C) Body weights were monitored throughout the experiment. (D) Platelets, (E) WBC counts, and (F) liver enzymes (shown are ALP, alanine transferase [ALT], and aspartate transferase [AST] levels) were measured on day 5 (Debio 1562 experiments), day 11 (Debio 1562M single dosing experiments), or day 23 (Debio 1562M Q3W ×2 dosing); two-way ANOVA p values: ∗∗ p < 0.01, ∗∗∗ p < 0.001, ∗∗∗∗ p < 0.0001. (G) PBMCs from healthy volunteers were cultured in the presence of vehicle or 1 μM Debio 1562M for 72 h prior to immunophenotyping and assessment of viability. n = 5; mean ± SEM. Two-way ANOVA p values: ∗ p < 0.05, ns: not significant. See also .

Article Snippet: Cryopreserved PBMCs from healthy donors were provided and handled by Eurofins Discovery.

Techniques: In Vivo, Clinical Proteomics, Injection, Control, Cell Culture

Journal: Cell Reports Medicine

Article Title: Debio 1562M CD37-targeting ADC is highly active and well tolerated in preclinical models of AML and MDS

doi: 10.1016/j.xcrm.2026.102749

Figure Lengend Snippet:

Article Snippet: Cryopreserved PBMCs from healthy donors were provided and handled by Eurofins Discovery.

Techniques: Recombinant, Proliferation Assay, Antibody Labeling, Software